Circulomics, a Baltimore, MD-based developer of a single molecule analysis system, received a $1.5M SBIR Phase II grant by the National Institutes of Health (NIH)/National Institute of General Medical Sciences (NIGMS).
The award will enable the company to develop a single molecule platform for DNA/RNA sample quality analysis based on PicoSep technology.
Led by CEO Dr. Kelvin Liu, Circulomics is advancing PicoSep, a single molecule instrument that can perform accurate sizing and quantification of DNA/RNA while consuming only picoliters of sample and 20 molecules of DNA.
Sizing occurs through a simple hydrodynamic mechanism based on the flow behavior of DNA in small buffer-filled microcapillaries. This mechanism allows for rapid and accurate sizing of DNA/RNA across a wide range of sizes including small DNA oligonucleotides and high molecular weight DNA. Quantification is performed by direct single molecule counting of the size-separated DNA.
The first products to be developed will be kits for RNA/DNA integrity analysis and high molecular weight DNA sizing. The sensitivity of single molecule counting will enable detection of minute amounts of degradation. Beyond these first products, PicoSep can be combined with novel molecular assays to examine other nucleic acid characteristics such as conformation and quality.